mouse anti-chicken cd3 (clone ct-3) (SouthernBiotech)
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Mouse Anti Chicken Cd3 (Clone Ct 3), supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-chicken+cd3+(clone+ct-3)/mouse+anti+chicken+cd4+fitc/pmc11832469-0-0-14
Average 90 stars, based on 1 article reviews
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1) Product Images from "Generation and characterization of chicken monocyte-derived dendritic cells"
Article Title: Generation and characterization of chicken monocyte-derived dendritic cells
Journal: Frontiers in Immunology
doi: 10.3389/fimmu.2025.1517697
Figure Legend Snippet: List of antibodies.
Techniques Used: Control
Figure Legend Snippet: Analysis of monocyte population enrichment following plastic adherence of PBMCs. (A) Gating strategy for flow cytometry analysis of CD3+ and MRC1LB+ cells. The mononuclear cell population was gated based on forward scatter (FSC)-A and side scatter (SSC)-A parameters and singlets were selected from the FSC-A versus FSC-H. Dead cells were excluded using a viability dye (B) Representative flow cytometry dot plots of CD3 expression by live PBMCs and adherent PBMCs (n = 3). Numbers indicate the percentage of positive cells.
Techniques Used: Flow Cytometry, Expressing
Figure Legend Snippet: LPS-stimulated chicken MoDCs induced the proliferation of autologous T cells. Splenocytes from naïve chickens were isolated and CD4 T cells were enriched by magnetic selection. The CD4 T cells were then CFSE-labelled and co-cultured with allogeneic unstimulated or 6 h LPS-stimulated MoDCs. (A) Flow cytometry gating strategy. CFSE-stained cells were selected. The mononuclear cell population was gated based on FSC-A and SCC-A parameters and singlets were selected from the FSC-A versus FSC-H. Dead cells were excluded using a viability dye and CD3+ cells were gated based on CD3 versus FSC-H. (B) Representative histograms of unstimulated and LPS-stimulated CFSE-labeled T cells proliferation. Numbers on histograms indicate the percentage of proliferating T cells. (C) Proportions of proliferating T cells. A two-tailed paired t-test was used to determine statistical differences (*p<0.01). The data from two independent experiments are displayed (n=8).
Techniques Used: Isolation, Selection, Cell Culture, Flow Cytometry, Staining, Labeling, Two Tailed Test
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