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mouse anti-chicken cd3 (clone ct-3)  (SouthernBiotech)


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    Structured Review

    SouthernBiotech mouse anti-chicken cd3 (clone ct-3)
    List of antibodies.
    Mouse Anti Chicken Cd3 (Clone Ct 3), supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti-chicken cd3 (clone ct-3)/product/SouthernBiotech
    Average 90 stars, based on 1 article reviews
    mouse anti-chicken cd3 (clone ct-3) - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Generation and characterization of chicken monocyte-derived dendritic cells"

    Article Title: Generation and characterization of chicken monocyte-derived dendritic cells

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2025.1517697

    List of antibodies.
    Figure Legend Snippet: List of antibodies.

    Techniques Used: Control

    Analysis of monocyte population enrichment following plastic adherence of PBMCs. (A) Gating strategy for flow cytometry analysis of CD3+ and MRC1LB+ cells. The mononuclear cell population was gated based on forward scatter (FSC)-A and side scatter (SSC)-A parameters and singlets were selected from the FSC-A versus FSC-H. Dead cells were excluded using a viability dye (B) Representative flow cytometry dot plots of CD3 expression by live PBMCs and adherent PBMCs (n = 3). Numbers indicate the percentage of positive cells.
    Figure Legend Snippet: Analysis of monocyte population enrichment following plastic adherence of PBMCs. (A) Gating strategy for flow cytometry analysis of CD3+ and MRC1LB+ cells. The mononuclear cell population was gated based on forward scatter (FSC)-A and side scatter (SSC)-A parameters and singlets were selected from the FSC-A versus FSC-H. Dead cells were excluded using a viability dye (B) Representative flow cytometry dot plots of CD3 expression by live PBMCs and adherent PBMCs (n = 3). Numbers indicate the percentage of positive cells.

    Techniques Used: Flow Cytometry, Expressing

    LPS-stimulated chicken MoDCs induced the proliferation of autologous T cells. Splenocytes from naïve chickens were isolated and CD4 T cells were enriched by magnetic selection. The CD4 T cells were then CFSE-labelled and co-cultured with allogeneic unstimulated or 6 h LPS-stimulated MoDCs. (A) Flow cytometry gating strategy. CFSE-stained cells were selected. The mononuclear cell population was gated based on FSC-A and SCC-A parameters and singlets were selected from the FSC-A versus FSC-H. Dead cells were excluded using a viability dye and CD3+ cells were gated based on CD3 versus FSC-H. (B) Representative histograms of unstimulated and LPS-stimulated CFSE-labeled T cells proliferation. Numbers on histograms indicate the percentage of proliferating T cells. (C) Proportions of proliferating T cells. A two-tailed paired t-test was used to determine statistical differences (*p<0.01). The data from two independent experiments are displayed (n=8).
    Figure Legend Snippet: LPS-stimulated chicken MoDCs induced the proliferation of autologous T cells. Splenocytes from naïve chickens were isolated and CD4 T cells were enriched by magnetic selection. The CD4 T cells were then CFSE-labelled and co-cultured with allogeneic unstimulated or 6 h LPS-stimulated MoDCs. (A) Flow cytometry gating strategy. CFSE-stained cells were selected. The mononuclear cell population was gated based on FSC-A and SCC-A parameters and singlets were selected from the FSC-A versus FSC-H. Dead cells were excluded using a viability dye and CD3+ cells were gated based on CD3 versus FSC-H. (B) Representative histograms of unstimulated and LPS-stimulated CFSE-labeled T cells proliferation. Numbers on histograms indicate the percentage of proliferating T cells. (C) Proportions of proliferating T cells. A two-tailed paired t-test was used to determine statistical differences (*p<0.01). The data from two independent experiments are displayed (n=8).

    Techniques Used: Isolation, Selection, Cell Culture, Flow Cytometry, Staining, Labeling, Two Tailed Test



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    Image Search Results


    List of antibodies.

    Journal: Frontiers in Immunology

    Article Title: Generation and characterization of chicken monocyte-derived dendritic cells

    doi: 10.3389/fimmu.2025.1517697

    Figure Lengend Snippet: List of antibodies.

    Article Snippet: Mouse anti-chicken CD3 (Clone CT-3) , IgG1κ , PACBLU (Pacific BlueTM) , 8200-26 , Southern Biotech , 20 μg/mL.

    Techniques: Control

    Analysis of monocyte population enrichment following plastic adherence of PBMCs. (A) Gating strategy for flow cytometry analysis of CD3+ and MRC1LB+ cells. The mononuclear cell population was gated based on forward scatter (FSC)-A and side scatter (SSC)-A parameters and singlets were selected from the FSC-A versus FSC-H. Dead cells were excluded using a viability dye (B) Representative flow cytometry dot plots of CD3 expression by live PBMCs and adherent PBMCs (n = 3). Numbers indicate the percentage of positive cells.

    Journal: Frontiers in Immunology

    Article Title: Generation and characterization of chicken monocyte-derived dendritic cells

    doi: 10.3389/fimmu.2025.1517697

    Figure Lengend Snippet: Analysis of monocyte population enrichment following plastic adherence of PBMCs. (A) Gating strategy for flow cytometry analysis of CD3+ and MRC1LB+ cells. The mononuclear cell population was gated based on forward scatter (FSC)-A and side scatter (SSC)-A parameters and singlets were selected from the FSC-A versus FSC-H. Dead cells were excluded using a viability dye (B) Representative flow cytometry dot plots of CD3 expression by live PBMCs and adherent PBMCs (n = 3). Numbers indicate the percentage of positive cells.

    Article Snippet: Mouse anti-chicken CD3 (Clone CT-3) , IgG1κ , PACBLU (Pacific BlueTM) , 8200-26 , Southern Biotech , 20 μg/mL.

    Techniques: Flow Cytometry, Expressing

    LPS-stimulated chicken MoDCs induced the proliferation of autologous T cells. Splenocytes from naïve chickens were isolated and CD4 T cells were enriched by magnetic selection. The CD4 T cells were then CFSE-labelled and co-cultured with allogeneic unstimulated or 6 h LPS-stimulated MoDCs. (A) Flow cytometry gating strategy. CFSE-stained cells were selected. The mononuclear cell population was gated based on FSC-A and SCC-A parameters and singlets were selected from the FSC-A versus FSC-H. Dead cells were excluded using a viability dye and CD3+ cells were gated based on CD3 versus FSC-H. (B) Representative histograms of unstimulated and LPS-stimulated CFSE-labeled T cells proliferation. Numbers on histograms indicate the percentage of proliferating T cells. (C) Proportions of proliferating T cells. A two-tailed paired t-test was used to determine statistical differences (*p<0.01). The data from two independent experiments are displayed (n=8).

    Journal: Frontiers in Immunology

    Article Title: Generation and characterization of chicken monocyte-derived dendritic cells

    doi: 10.3389/fimmu.2025.1517697

    Figure Lengend Snippet: LPS-stimulated chicken MoDCs induced the proliferation of autologous T cells. Splenocytes from naïve chickens were isolated and CD4 T cells were enriched by magnetic selection. The CD4 T cells were then CFSE-labelled and co-cultured with allogeneic unstimulated or 6 h LPS-stimulated MoDCs. (A) Flow cytometry gating strategy. CFSE-stained cells were selected. The mononuclear cell population was gated based on FSC-A and SCC-A parameters and singlets were selected from the FSC-A versus FSC-H. Dead cells were excluded using a viability dye and CD3+ cells were gated based on CD3 versus FSC-H. (B) Representative histograms of unstimulated and LPS-stimulated CFSE-labeled T cells proliferation. Numbers on histograms indicate the percentage of proliferating T cells. (C) Proportions of proliferating T cells. A two-tailed paired t-test was used to determine statistical differences (*p<0.01). The data from two independent experiments are displayed (n=8).

    Article Snippet: Mouse anti-chicken CD3 (Clone CT-3) , IgG1κ , PACBLU (Pacific BlueTM) , 8200-26 , Southern Biotech , 20 μg/mL.

    Techniques: Isolation, Selection, Cell Culture, Flow Cytometry, Staining, Labeling, Two Tailed Test