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mouse anti-chicken cd3 (clone ct-3)  (SouthernBiotech)


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    Structured Review

    SouthernBiotech mouse anti-chicken cd3 (clone ct-3)
    List of antibodies.
    Mouse Anti Chicken Cd3 (Clone Ct 3), supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti-chicken+cd3+(clone+ct-3)/mouse+anti+chicken+cd4+fitc/pmc11832469-0-0-14
    Average 90 stars, based on 1 article reviews
    mouse anti-chicken cd3 (clone ct-3) - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Generation and characterization of chicken monocyte-derived dendritic cells"

    Article Title: Generation and characterization of chicken monocyte-derived dendritic cells

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2025.1517697

    List of antibodies.
    Figure Legend Snippet: List of antibodies.

    Techniques Used: Control

    Analysis of monocyte population enrichment following plastic adherence of PBMCs. (A) Gating strategy for flow cytometry analysis of CD3+ and MRC1LB+ cells. The mononuclear cell population was gated based on forward scatter (FSC)-A and side scatter (SSC)-A parameters and singlets were selected from the FSC-A versus FSC-H. Dead cells were excluded using a viability dye (B) Representative flow cytometry dot plots of CD3 expression by live PBMCs and adherent PBMCs (n = 3). Numbers indicate the percentage of positive cells.
    Figure Legend Snippet: Analysis of monocyte population enrichment following plastic adherence of PBMCs. (A) Gating strategy for flow cytometry analysis of CD3+ and MRC1LB+ cells. The mononuclear cell population was gated based on forward scatter (FSC)-A and side scatter (SSC)-A parameters and singlets were selected from the FSC-A versus FSC-H. Dead cells were excluded using a viability dye (B) Representative flow cytometry dot plots of CD3 expression by live PBMCs and adherent PBMCs (n = 3). Numbers indicate the percentage of positive cells.

    Techniques Used: Flow Cytometry, Expressing

    LPS-stimulated chicken MoDCs induced the proliferation of autologous T cells. Splenocytes from naïve chickens were isolated and CD4 T cells were enriched by magnetic selection. The CD4 T cells were then CFSE-labelled and co-cultured with allogeneic unstimulated or 6 h LPS-stimulated MoDCs. (A) Flow cytometry gating strategy. CFSE-stained cells were selected. The mononuclear cell population was gated based on FSC-A and SCC-A parameters and singlets were selected from the FSC-A versus FSC-H. Dead cells were excluded using a viability dye and CD3+ cells were gated based on CD3 versus FSC-H. (B) Representative histograms of unstimulated and LPS-stimulated CFSE-labeled T cells proliferation. Numbers on histograms indicate the percentage of proliferating T cells. (C) Proportions of proliferating T cells. A two-tailed paired t-test was used to determine statistical differences (*p<0.01). The data from two independent experiments are displayed (n=8).
    Figure Legend Snippet: LPS-stimulated chicken MoDCs induced the proliferation of autologous T cells. Splenocytes from naïve chickens were isolated and CD4 T cells were enriched by magnetic selection. The CD4 T cells were then CFSE-labelled and co-cultured with allogeneic unstimulated or 6 h LPS-stimulated MoDCs. (A) Flow cytometry gating strategy. CFSE-stained cells were selected. The mononuclear cell population was gated based on FSC-A and SCC-A parameters and singlets were selected from the FSC-A versus FSC-H. Dead cells were excluded using a viability dye and CD3+ cells were gated based on CD3 versus FSC-H. (B) Representative histograms of unstimulated and LPS-stimulated CFSE-labeled T cells proliferation. Numbers on histograms indicate the percentage of proliferating T cells. (C) Proportions of proliferating T cells. A two-tailed paired t-test was used to determine statistical differences (*p<0.01). The data from two independent experiments are displayed (n=8).

    Techniques Used: Isolation, Selection, Cell Culture, Flow Cytometry, Staining, Labeling, Two Tailed Test

    Related Articles

    Bioprocessing:

    Article Title: Effects of Selected Prebiotics or Synbiotics Administered in ovo on Lymphocyte Subsets in Bursa of the Fabricius, Thymus, and Spleen in Non-Immunized and Immunized Chicken Broilers
    Article Snippet: .. The following monoclonal antibodies (SouthernBiotech, Birmingham, AL, USA) were used in the experiment: Mouse Anti-Chicken Bu-1-FITC (Clone AV20), Mouse Anti-Chicken CD3-R-PE (Clone CT-3), Mouse Anti-Chicken TCRγδ-FITC (Clone TCR-1), Mouse Anti-Chicken CD4-FITC (Clone CT-4), and Mouse Anti-Chicken CD8α-CY-5 (Clone CT-8). ..

    other:

    Article Title: Immune cell profile and metabolic preference following intramuscular lipopolysaccharide injection of highly inbred and advanced intercross genetic lines
    Article Snippet: The flow cytometry panel included the following antibodies to detect innate immune cell and T lymphocyte populations: PE anti-chicken monocyte/macrophage (clone KUL01; mouse IgG1κ), Pacific BlueTM anti-chicken CD3 (clone CT-3; mouse IgG1κ), FITC anti-chicken CD1.1 (clone CB3; mouse IgG1κ), PE/CY7 anti-chicken CD4 (clone CT-4; mouse IgG1κ), Alexa Fluor ® 700 anti-chicken CD8α (clone CT-8; mouse IgG1κ; Southern Biotech, Birmingham, AL).

    Article Title: Development of novel reagents to chicken FLT3, XCR1 and CSF2R for the identification and characterization of avian conventional dendritic cells
    Article Snippet: Mouse anti‐CD3/ clone CT−3 , CD3 , RPE , Mouse IgG1 , Southern Biotech , 0·1.

    Article Title: Development and function of chicken XCR1 + conventional dendritic cells
    Article Snippet: Mouse anti-CD3/clone CT-3 , CD3 , Mouse IgG1 , R-PE, AF647 or Donkey anti-mouse IgG-AF594 , Southern Biotech.

    Article Title: Generation and characterization of chicken monocyte-derived dendritic cells
    Article Snippet: Mouse anti-chicken CD3 (Clone CT-3) , IgG1κ , PACBLU (Pacific BlueTM) , 8200-26 , Southern Biotech , 20 μg/mL.

    Staining:

    Article Title: Thymus-forming potential of the second pharyngeal pouch and its regulation by local mesenchyme in avian embryos.
    Article Snippet: .. Cells were stained using mouse anti-chicken mABs CD3-PE (clone CT-3; Southern Biotech) according to manufacturer’s instructions. .. Flow cytometry analysis was performed using LSR Fortessa cytometer (Becton Dickinson) and FlowJo v10 software (BD Biosciences).



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    Image Search Results


    List of antibodies.

    Journal: Frontiers in Immunology

    Article Title: Generation and characterization of chicken monocyte-derived dendritic cells

    doi: 10.3389/fimmu.2025.1517697

    Figure Lengend Snippet: List of antibodies.

    Article Snippet: Mouse anti-chicken CD3 (Clone CT-3) , IgG1κ , PACBLU (Pacific BlueTM) , 8200-26 , Southern Biotech , 20 μg/mL.

    Techniques: Control

    Analysis of monocyte population enrichment following plastic adherence of PBMCs. (A) Gating strategy for flow cytometry analysis of CD3+ and MRC1LB+ cells. The mononuclear cell population was gated based on forward scatter (FSC)-A and side scatter (SSC)-A parameters and singlets were selected from the FSC-A versus FSC-H. Dead cells were excluded using a viability dye (B) Representative flow cytometry dot plots of CD3 expression by live PBMCs and adherent PBMCs (n = 3). Numbers indicate the percentage of positive cells.

    Journal: Frontiers in Immunology

    Article Title: Generation and characterization of chicken monocyte-derived dendritic cells

    doi: 10.3389/fimmu.2025.1517697

    Figure Lengend Snippet: Analysis of monocyte population enrichment following plastic adherence of PBMCs. (A) Gating strategy for flow cytometry analysis of CD3+ and MRC1LB+ cells. The mononuclear cell population was gated based on forward scatter (FSC)-A and side scatter (SSC)-A parameters and singlets were selected from the FSC-A versus FSC-H. Dead cells were excluded using a viability dye (B) Representative flow cytometry dot plots of CD3 expression by live PBMCs and adherent PBMCs (n = 3). Numbers indicate the percentage of positive cells.

    Article Snippet: Mouse anti-chicken CD3 (Clone CT-3) , IgG1κ , PACBLU (Pacific BlueTM) , 8200-26 , Southern Biotech , 20 μg/mL.

    Techniques: Flow Cytometry, Expressing

    LPS-stimulated chicken MoDCs induced the proliferation of autologous T cells. Splenocytes from naïve chickens were isolated and CD4 T cells were enriched by magnetic selection. The CD4 T cells were then CFSE-labelled and co-cultured with allogeneic unstimulated or 6 h LPS-stimulated MoDCs. (A) Flow cytometry gating strategy. CFSE-stained cells were selected. The mononuclear cell population was gated based on FSC-A and SCC-A parameters and singlets were selected from the FSC-A versus FSC-H. Dead cells were excluded using a viability dye and CD3+ cells were gated based on CD3 versus FSC-H. (B) Representative histograms of unstimulated and LPS-stimulated CFSE-labeled T cells proliferation. Numbers on histograms indicate the percentage of proliferating T cells. (C) Proportions of proliferating T cells. A two-tailed paired t-test was used to determine statistical differences (*p<0.01). The data from two independent experiments are displayed (n=8).

    Journal: Frontiers in Immunology

    Article Title: Generation and characterization of chicken monocyte-derived dendritic cells

    doi: 10.3389/fimmu.2025.1517697

    Figure Lengend Snippet: LPS-stimulated chicken MoDCs induced the proliferation of autologous T cells. Splenocytes from naïve chickens were isolated and CD4 T cells were enriched by magnetic selection. The CD4 T cells were then CFSE-labelled and co-cultured with allogeneic unstimulated or 6 h LPS-stimulated MoDCs. (A) Flow cytometry gating strategy. CFSE-stained cells were selected. The mononuclear cell population was gated based on FSC-A and SCC-A parameters and singlets were selected from the FSC-A versus FSC-H. Dead cells were excluded using a viability dye and CD3+ cells were gated based on CD3 versus FSC-H. (B) Representative histograms of unstimulated and LPS-stimulated CFSE-labeled T cells proliferation. Numbers on histograms indicate the percentage of proliferating T cells. (C) Proportions of proliferating T cells. A two-tailed paired t-test was used to determine statistical differences (*p<0.01). The data from two independent experiments are displayed (n=8).

    Article Snippet: Mouse anti-chicken CD3 (Clone CT-3) , IgG1κ , PACBLU (Pacific BlueTM) , 8200-26 , Southern Biotech , 20 μg/mL.

    Techniques: Isolation, Selection, Cell Culture, Flow Cytometry, Staining, Labeling, Two Tailed Test